Reverse Transcription Polymerase Chain Reaction:Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: The Promega GoScript-brand RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA) instead of the random oligo mixture. (Promega Corporation, Madison, Wis.) Following the inactivation of the reverse transcriptase, the cDNA was purified using Qiagen PCR Purification kit. (Qiagen Inc., Germantown, Md.)
Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: The Promega GoScript-brand RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA) instead of the random oligo mixture. (Promega Corporation, Madison, WI) Following the inactivation of the reverse transcriptase, the cDNA was purified using Qiagen PCR Purification kit. (Qiagen Inc., Germantown, MD.)
Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: Polycistronic Verification Using the DNAse treated RNA isolated from LA grown P. putida KT2440, cDNA for the operon was generated with the Promega GoScript-brand RT PCR kit using 1 μL of a 10 μM gene specific oligo (JMR237).
Reverse Transcription:Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: The Promega GoScript-brand RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA) instead of the random oligo mixture. (Promega Corporation, Madison, Wis.) Following the inactivation of the reverse transcriptase, the cDNA was purified using Qiagen PCR Purification kit. (Qiagen Inc., Germantown, Md.)
Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: The Promega GoScript-brand RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA) instead of the random oligo mixture. (Promega Corporation, Madison, WI) Following the inactivation of the reverse transcriptase, the cDNA was purified using Qiagen PCR Purification kit. (Qiagen Inc., Germantown, MD.)
Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: Polycistronic Verification Using the DNAse treated RNA isolated from LA grown P. putida KT2440, cDNA for the operon was generated with the Promega GoScript-brand RT PCR kit using 1 μL of a 10 μM gene specific oligo (JMR237).
Purification:Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: The Promega GoScript-brand RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA) instead of the random oligo mixture. (Promega Corporation, Madison, Wis.) Following the inactivation of the reverse transcriptase, the cDNA was purified using Qiagen PCR Purification kit. (Qiagen Inc., Germantown, Md.)
Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: The Promega GoScript-brand RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA) instead of the random oligo mixture. (Promega Corporation, Madison, WI) Following the inactivation of the reverse transcriptase, the cDNA was purified using Qiagen PCR Purification kit. (Qiagen Inc., Germantown, MD.)
Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: Polycistronic Verification Using the DNAse treated RNA isolated from LA grown P. putida KT2440, cDNA for the operon was generated with the Promega GoScript-brand RT PCR kit using 1 μL of a 10 μM gene specific oligo (JMR237).
Polymerase Chain Reaction:Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: The Promega GoScript-brand RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA) instead of the random oligo mixture. (Promega Corporation, Madison, Wis.) Following the inactivation of the reverse transcriptase, the cDNA was purified using Qiagen PCR Purification kit. (Qiagen Inc., Germantown, Md.)
Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: The Promega GoScript-brand RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA) instead of the random oligo mixture. (Promega Corporation, Madison, WI) Following the inactivation of the reverse transcriptase, the cDNA was purified using Qiagen PCR Purification kit. (Qiagen Inc., Germantown, MD.)
Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: Polycistronic Verification Using the DNAse treated RNA isolated from LA grown P. putida KT2440, cDNA for the operon was generated with the Promega GoScript-brand RT PCR kit using 1 μL of a 10 μM gene specific oligo (JMR237).
Isolation:Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: The Promega GoScript-brand RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA) instead of the random oligo mixture. (Promega Corporation, Madison, Wis.) Following the inactivation of the reverse transcriptase, the cDNA was purified using Qiagen PCR Purification kit. (Qiagen Inc., Germantown, Md.)
Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: The Promega GoScript-brand RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA) instead of the random oligo mixture. (Promega Corporation, Madison, WI) Following the inactivation of the reverse transcriptase, the cDNA was purified using Qiagen PCR Purification kit. (Qiagen Inc., Germantown, MD.)
Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: Polycistronic Verification Using the DNAse treated RNA isolated from LA grown P. putida KT2440, cDNA for the operon was generated with the Promega GoScript-brand RT PCR kit using 1 μL of a 10 μM gene specific oligo (JMR237).
Generated:Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: The Promega GoScript-brand RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA) instead of the random oligo mixture. (Promega Corporation, Madison, Wis.) Following the inactivation of the reverse transcriptase, the cDNA was purified using Qiagen PCR Purification kit. (Qiagen Inc., Germantown, Md.)
Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: The Promega GoScript-brand RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA) instead of the random oligo mixture. (Promega Corporation, Madison, WI) Following the inactivation of the reverse transcriptase, the cDNA was purified using Qiagen PCR Purification kit. (Qiagen Inc., Germantown, MD.)
Article Title: Bioconversion of levulinic acid in genetically engineered hosts
Article Snippet: Polycistronic Verification Using the DNAse treated RNA isolated from LA grown P. putida KT2440, cDNA for the operon was generated with the Promega GoScript-brand RT PCR kit using 1 μL of a 10 μM gene specific oligo (JMR237).
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